Antibacterial Activity Test of the Combination of Binahong Leaf Extract (Anredera cordifolia (Ten.) Steenis) and Moringa Leaf Extract (Moringa oleifera Lam.) against Propionibacterium acnes, the Bacteria that Causes Acne
Research
DOI:
https://doi.org/10.55175/cdk.v52i12.1427Keywords:
Antibacterial, binahong leaves, moringa leaves, acne, Propionibacterium acnesAbstract
Introduction: Acne is a skin disease that is often found in many teenagers to young adults. Propionibacterium acnes is one of the causes of acne infection with a prevalence of 12.9%. As long-term inappropriate use of antibiotics can cause resistance, traditional medicine using natural ingredients can act as an alternative. The secondary metabolite compounds from binahong (Anredera cordifolia (Ten.) Steenis) leaves and moringa (Moringa oleifera Lam.) leaves are believed to have antibacterial activity. This study aims to determine the antibacterial activity of a combination of binahong leaves and moringa leaves in inhibiting the growth of acne-causing bacteria, Propionibacterium acnes. Methods: This quantitative experimental research study tested the antibacterial properties of binahong leaves and moringa leaves against
Propionibacterium acne with random design (RAL) of 11 treatments with 3 repetitions. Bacterial test used paper disc diffusion with a single concentration of 20%, 40%, 60% and a combination of 20%:20%, 40%:60%, and 60%:40%, with clindamycin as positive control and sterile aquadest as negative control. Results: The phytochemical screening results were that binahong leaves and moringa leaves contained alkaloids, tannins, saponins, and flavonoids while the results of the antibacterial activity test were that the 60%:40% combination was more effective in inhibiting the growth of Propionibacterium acnes with an inhibition zone diameter of 26.7 mm ± 2.0 (strong). Conclusion: Binahong leaves and Moringa leaves contain secondary metabolite compounds of tannins, alkaloids, saponins, and flavonoids, and have antibacterial activity. Binahong leaves and moringa leaves can inhibit the growth of bacterium Propionibacterium acnes with a strong inhibition zone category.
Downloads
References
Sibero HT, Sirajudin A, Anggraini D. Prevalensi dan gambaran epidemiologi acne vulgaris di provinsi Lampung. J Farm Komunitas.2019;3(2):62–8. https://doi.org/10.23960/jkunila.v3i2.pp308-312.
Riswana AP, Indriarini D, Agnes M, Dedy E. Uji aktivitas antibakteri ekstrak daun kelor (Moringa oleifera) terhadap pertumbuhan bakteri penyebab jerawat. Seminar Nasional Riset Kedokteran (SENSORIK) 2022;(3):50−62.
Sasebohe VY, Prakasita VC, Aditiyarini D. Aktivitas antibakteri ekstrak etanol daun binahong terhadap Staphylococcus aureus dan Propionibacterium acnes penyebab jerawat. Sciscitatio. 2023;4(1):1−14
Chairunnisa A, Masruriati E, Ariyanti. Efektivitas gel ekstrak etanol 70% daun kelor terhadap Propionibacterium acnes. Pharmaceut Traditional Med. 2017;1(2):64–72.
Pariury JA, Herman JPC, Rebecca T, Veronica E, Arijana IGKN. Potensi kulit jeruk Bali (Citrus maxima Merr) sebagai antibakteri
Propionibacterium acne penyebab jerawat. Hang Tuah Med J. 2021;19(1):119–31. https://doi.org/10.30649/htmj.v19i1.65.
Khairina SR, Azzahro RF, Shafiyyah S. Anti-bacterial ointment with binahong leaf extract. J Beauty Cosmetol. 2022;3(2):13–6. https://doi.org/10.26740/jbc.v3n2.p13-16.
Wahyuningsih ES, Sumaryono W, Chaidir. Aktivitas kombinasi ekstrak daun kelor (Moringa Oleifera L) dan ekstrak daun sirih merah (Piper Crocatum) sebagai antijerawat pembuatan serbuk simplisia. J Pharmacopolium. 2021;4(3):123–9.
Syamsul ES, Anugrah O, Supriningrum R. Penetapan rendemen ekstrak daun jambu mawar (Syzygium jambos L. Alston) berdasarkan variasi konsentrasi etanol dengan metode maserasi. J Ris Kefarmasian Indones. 2020;2(3):147–57. doi:10.33759/jrki.v2i3.98
Nurjannah I, Mustariani BAA, Suryani N. Skrining fitokimia dan uji antibakteri ekstrak kombinasi daun jeruk purut (Citrus hystrix) dan kelor (Moringa oleifera L.) sebagai zat aktif pada sabun antibakteri. SPIN J Kim Pendidik Kim. 2022;4(1):23–36. doi:10.20414/spin.v4i1.4801.
Meigaria KM, Mudianta IW, Martiningsih NW. Skrining fitokimia dan uji aktivitas antioksidan ekstrak aseton daun kelor (Moringa oleifera). J Wahana Mat Sains. 2016;10(1):1–11.
Bahri S, Akhatik N, Sumantri NR. Uji aktivitas antibakteri ekstrak etanol daun jagung manis (Zea mays var. saccharata (Sturtev.) L. H.Bailey) terhadap pertumbuhan bakteri penyebab jerawat (Staphylococcus epidermidis dan Propionibacterium acnes) antibacterial.Arch Pharm. 2022;4(2): 63−77.
Wendersteyt NV, Wewengkang DS, Abdullah SS. Uji aktivitas antimikroba dari ekstrak dan fraksi Ascidian Herdmania Momus dari perairan pulau Bangka Likupang terhadap pertumbuhan mikroba Staphylococcus aureus, Salmonella typhimurium dan Candida albicans. Pharmacon. 2021;10(1):706. https://doi.org/10.35799/pha.10.2021.32758.
Kementrian Kesehatan RI. Farmakope herbal Indonesi edisi II. Jakarta: Kementrian Kesehatan RI; 2017.
Subaryanti, Meianti DSD, Manalu R. Potensi antimikroba ekstrak etanol daun gatal (Urticastrum decumanum ( Roxb .) Kuntze ) terhadap pertumbuhan Staphylococcus aureus dan Candida albicans. J Ilmu Kefarmasian (Saintech Farma). 2022;15(2):93–102. https://doi.org/10.37277/sfj.v15i2.1272.
Surbakti PAA, Queljoe E De, Boddhi W. Skrining fitokimia dan uji toksisitas ekstrak etanol daun binahong (Andredera cordifolia (Ten.) Steenis) dengan metode brine shrimp lethality test (BSLT). Pharmacon. 2018;7(3):22–31. https://doi.org/10.35799/pha.7.2018.20112.
Hanifah, Anjani TP. Skrining fitokimia daun binahong (Anredera cordifolia) dari kabupaten Semarang yang diekstrak menggunakan pelarut air. J Aquat Asia 2022;7(2):99–103.
Munira M, Amalia D, Khazanah W, Nasir M, Farmasi S, Kesehatan P, et al. Uji aktivitas antibakteri ekstrak etanol daun kelor (Moringa oleifera Lamk) berdasarkan waktu panen . Indones J Heal Sci. 2021;5(2):69–76. https://doi.org/10.24269/ijhs.v5i2.3640.
Sariwating M, Umar CBP, Buamona WS. Skrining fitokimia dan uji daya hambat ekstrak etanol daun binahong (Anredera cordifolia) asal sanana terhadap pertumbuhan bakteri Propionibacterium acnes. J Ris Rumpun Ilmu Kesehat. 2022;1(1):150–9. https://doi.org/10.55606/jurrikes.v1i1.898.
Oktavia FD, Sutoyo S. Skrining fitokimia, kandungan flavonoid total, dan aktivitas antioksidan ekstrak etanol tumbuhan Selaginella doederleinii. J Kim Ris. 2021;6(2):141. https://doi.org/10.20473/jkr.v6i2.30904.
Fitriani OS, Putra FA, Yesti Y, Saputra HA, Wirasti N. Potensi ekstrak daun kelor (Moringa oleifera Lam) dalam menghambat pertumbuhan bakteri (Propionibacterium acnes). Hum Care J. 2023;8(2):291–7.
Seran F, Jasmanindar Y, Solosso Y. Uji fitokimia dan aktivitas antibakteri daun binahong (Anredera cordifolia) terhadap bakteri Vibro alginolyticus in-vitro. J Aquatik. 2022;5(1):1–8. https://doi.org/10.35508/aquatik.v5i1.6536.
Ap AT, Maria C, Susanti E, Azis A, Rasyid A, Weno I, et al. Kandungan kualitatif senyawa metabolit sekunder ekstrak etanol daun pandemor (Pemphis acidula JR Forst & G Forst) asal pulau Biak. J Kehut Papuasi. 2022;8(1):47–54. doi: 10.46703/jurnalpapuasia.Vol8. Iss1.289.
Amin SS, Ghozali TZ, Efendi MRS. Identifikasi bakteri dari telapak tangan dengan pewarnaan gram. J Kimia dan Ilmu Lingkung. 2023;1(1):30–5. https://doi.org/10.56071/chemviro.v1i1.563.
Baraga PV, Mahyarudin M, Rialita A. Aktivitas antibakteri metabolit sekunder isolat bakteri endofit kunyit (Curcuma longa L.) terhadap Propionibacterium acnes. Bioma J Ilmu Biol. 2022;11(1):103–20. https://doi.org/10.26877/bioma.v11i1.10558.
Gerung WHP, Fatimawali, Antasionasti I. Uji aktivitas antibakteri ekstrak daun belimbing botol (Averrhoa bilimbi L.) terhadap pertumbuhan bakteri Propionibacterium acnes Penyebab Jerawat. Pharmacon. 2021;10(4):1087–93. https://doi.org/10.35799/pha.10.2021.37403.
Puspa Anggraeni Putri, Chatri M, Advinda L, Violita. Karakteristik saponin senyawa metabolit sekunder pada tumbuhan. Serambi Biologi. 2023;8(2):251–8.
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2025 mela agustina_11 agustin_08
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
