Peranan Ekstrak Solanum lycopersicum L terhadap Ekspresi Basic Fibroblast Growth Factors (bFGF) dan Pencegahan Skar Hipertrofik pada Tikus Strain Wistar
DOI:
https://doi.org/10.55175/cdk.v46i5.464Keywords:
basic fibroblast growth factors, skar hipertrofik, Solanum lycopersicumAbstract
Tujuan. Mengetahui peranan ekstrak Solanum lycopersicum L (tomat) pada ekspresi basic fibroblast growth factor (bFGF) dan mencegah skar hipertrofik. Metode. Penelitian eksperimental laboratorium dengan rancangan post-test only control group design di Laboratorium Bagian Farmasi Universitas Setia Budi selama 28 hari menggunakan 30 ekor hewan coba tikus strain Wistar yang dibagi 3 kelompok. Pada seluruh tikus dibuat perlukaan di area punggung. Kelompok 1 dengan perlakuan Solanum lycopersicum L 20% gel topikal. Kelompok 2 dengan perlakuan topikal gel transparan (berisi kombinasi karboksimetilselulosa 2,3% dan propilen glikol 20%). Kelompok 3 tanpa perlakuan. Penilaian ekspresi bFGF berdasarkan pemeriksaan imunohistokimia dengan vectastain. Penilaian scar elevation index (SEI) berdasarkan pemeriksaan histopatologi dengan pewarnaan hematoksilin-eosin (HE) di Laboratorium Patologi Anatomi Fakultas Kedokteran Universitas Sebelas Maret Surakarta. Hasil. Tidak ada perbedaan bermakna ekspresi bFGF pada ketiga kelompok. Didapatkan perbedaan bermakna penilaian SEI; di kelompok 1 dengan perlakuan Solanum lycopersicum L 20% gel topikal tidak terbentuk skar hipertrofik.
Objective: To observe the role of Solanum lycopersicum L (tomato) extract on basic fibroblast growth factor (bFGF) expression and in hypertrophic scarring prevention. Method. A laboratory experimental research with post-test only control group design, conducted at the Pharmacy Section, Setia Budi University, on 30 Wistar strain rats divided into 3 groups. All rats were injured in the back area. Group 1 were treated with topical Solanum lycopersicum L 20% gel. Group 2 were treated with topical transparent gel (contain carboxymethylcellulose polymer 2,3 % and propylene glycol 20%). Group 3 without any treatment, all for 28 days. bFGF expression was assessed immunohistochemically with vectastain; scar elevation index (SEI) was histopathologically assessed with hemotoxicillin-eosin (HE) staining in Anatomy Pathology Laboratory of Faculty of Medical Sebelas Maret University. Results. No significant differences in bFGF expression in the three groups. SEI assessment analysis also showed no significant differences; owever, no hypertrophic scars formed in group
Downloads
References
Pusponegoro AD. Luka. In: Sjamsuhidajat R, De JongW, eds. Buku ajar ilmu bedah. 2nd Ed. Jakarta: EGC; 2005. p. 66-88
Grazul-Bilska AT, Johnson ML, Bilski JJ, Redmer DA, Reynolds LP, Abdullah A, et al. Wound healing: The role of growth factors. Drugs of today. 2003; 39(10):787-800
Song R, Bian HN, Lai W, Chen HD, Zhao KS. Normal skin and hypertrophic scar fibroblasts differentially regulate collagen and fibronectin expression as well as mitochondrial membrane potential in response to basic fibroblast growth factor. Braz J Med Biol Res. 2011;44(5):402-10
Shroff A, Mamalis A, Jagdeo J. Oxidative stress and skin fibrosis. Curr Pathobiol Rep. 2014;2:257-67
Kurahashi T, Fujii J. Role of antioxidant enzymes in wound healing. J Development Biol. 2015;3:1-14
Uthairatanakij A, Aiamla, S, Jitareerat, P, Maneenoi A. A preliminary comparison of antioxidants of tomato fruit grown under organic and conventional systems. Horticulturae. 2017;3(21):1-6
Feng K, Yu J, Cheng Y, Ruan M, Wang R, Ye Q, et al. The SOD gene family in tomato: identification, phylogenetic relationships and expression patterns. Frontiers in Plant Sci. 2016;7:1-12
Zamide EEI, Ajani RS, Oladopo OO. Does lycopersicon esculentum (Tomato) accelerate or retard wound healing in Wistar rats. Anat J Africa. 2015;4(1):522-7
Le Quéré S, Lacan, D, Lemaire B, Carillon, J, Schmitt, K. The role of superoxide dismutase (SOD) in skin disorders: A review. Nutrafoods. 2014;13:13-27
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2019 https://creativecommons.org/licenses/by-nc/4.0/
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.