Antigen untuk Metode Serologi Deteksi Antibodi Anti-HIV
DOI:
https://doi.org/10.55175/cdk.v50i9.722Kata Kunci:
Antigen, EIA, peptida, protein, uji serologiAbstrak
HIV dapat ditetapkan diagnosisnya dengan berbagai metode. Berbagai metode uji serologi antara lain EIA yang telah dikembangkan dari generasi satu sampai generasi keempat sampai uji cepat deteksi antibodi anti-HIV. Deteksi antibodi spesifik patogen menggunakan antigen tertentu yang berasal dari patogen tersebut. Antigen bisa berasal dari protein baik hasil isolasi maupun protein rekombinan, dan/ atau menggunakan peptida baik rekombinan maupun sintetik.
HIV can be diagnosed with varieties of the methods. Various serological test methods such as EIA have been developed from the first to the fourth generation to rapid test to detect anti-HIV antibody. Pathogen-specific antibodies can be detected by using a specific antigen derived from the pathogen. Antigens may originate from either isolate proteins or recombinant proteins, and or use either recombinant or synthetic peptides.
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Referensi
Kementerian Kesehatan Republik Indonesia. Peraturan Menteri Kesehatan Republik Indonesia nomor 15 tahun 2015 tentang pelayanan laboratorium pemeriksa HIV dan infeksi oportunistik. 2015.
Mehra B, Bhattar S, Bhalla P, Rawat D. Rapid tests versus ELISA for screening of HIV infection: Our experience from a voluntary counselling and testing facility of a tertiary care centre in North India. ISRN AIDS Hindawi. 2014;2014:296840.
Tiwari RP, Jain A, Khan Z, Kumar P, Bhrigu V, Bisen PS. Designing of novel antigenic peptide cocktail for the detection of antibodies to HIV-1/2 by ELISA. J Immunol Methods 2013;387(1–2):157–66.
Shetty S, Prabhu S. Laboratory tests for HIV: Diagnosing, monitoring and managing AIDS-an overview. Int J Oral & Maxillofacial Patho. 2011;2(1):20–8.
Kemenkes RI. Pedoman nasional tes dan konseling HIV dan AIDS. Jakarta; 2013.
Mcmichael AJ, Borrow P, Tomaras GD, Goonetilleke N, Haynes BF. The immune response during acute HIV-1 infection: Clues for vaccine development. Nat Rev Immunol. 2010;10(1):11–23.
Lampejo T, Pillay D. HIV virology, testing and monitoring. Med (United Kingdom). 2013;41(8):420–4.
Cornett JK, Kirn TJ. Laboratory diagnosis of HIV in adults: A review of current methods. Clin Infect Dis. 2013;57(5):712–8.
Branson BM. The future of HIV prevention. J Acquir Immune Defic Syndr. 2010;55(Supplement 2):102–5.
Daskalakis D. HIV diagnostic testing: Evolving technology and testing strategies. Top Antivir Med. 2011;19(1):18–22.
Speers D, Phillips P, Dyer J. Combination assay detecting both human immunodeficiency virus (HIV) p24 antigen and anti-HIV antibodies opens a second diagnostic window. J Clin Microbiol. 2005;43(10):5397.
Saville RD, Constantine NT, Cleghorn FR, Jack N, Bartholomew C, Edwards J, et al. Fourth-generation enzyme-linked immunosorbent assay for the simultaneous detection of human immunodeficiency virus antigen and antibody. J Clin Microbiol. 2001;39(7):2518–24.
Alexander TS. Human immunodeficiency virus diagnostic testing : 30 Years of evolution. Clin Vaccines Immunol. 2016;23(4):249–53.
Lien TX, Tien NTK, Chanpong GF, Cuc CT, Yen VT, Soderquist R, et al. Evaluation of rapid diagnostic tests for the detection of human immunodeficiency virus types 1 and 2, hepatitis B surface antigen, and syphilis in Ho Chi Minh City, Vietnam. Am J Trop Med Hyg. 2000;62(2):301-9.
Almazini P. Rapid test makin cepat mendeteksi antibodi HIV. CDK. 2011;38(2):137–40.
Greenwald JL, Burstein GR, Pincus J, Branson B. A rapid review of rapid HIV antibody tests. Curr Infect Dis Rep. 2006;8:125-31.
Delaney KP, Branson BM, Uniyal A, Phillips S, Candal D, Owen SM, et al. Evaluation of the performance characteristics of 6 rapid HIV antibody tests. CID. 2011;52(15 January):257–63.
Branson BM, Owen SM, Wesolowski LG, Benett B, Werner BG, Wroblewski KE, et al. Laboratory testing for the diagnosis of HIV infection : Updated recommendations - Guidelines and recommendations. Centers for Disease Control and Prevention and Association of Public Health Laboratories; 2014. p. 1-66.
Alonso R, Roa PL, Suárez M, Bouza E. New automated chemiluminescence immunoassay for simultaneous but separate detection of human immunodeficiency virus antigens and antibodies. J Clin Microbiol. 2014;52(5):1467–70.
Bio-Rad Laboratories. BioPlex 2200 system: HIV Ag-Ab, Instructions for use. USER MANUAL. 2015;38.
Movahedi A (R ), Hampson DJ. New ways to identify novel bacterial antigens for vaccine development. Vet Microbiol. 2008;131(1–2):1–13.
Structural Genomics Consortium, Architecture et Fonction des Macromolécules Biologiques, Berkeley Structural Genomics Center, China Structural Genomics Consortium, Integrated Center for Structure and Function Innovation, Israel Structural Proteomics Cente and S-C. Protein production and purification. Nat Methods. 2008;5(2):135-46.
Carmona SJ, Sartor PA, Leguizamón MS, Campetella OE, Agüero F. Diagnostic peptide discovery: Prioritization of pathogen diagnostic markers using multiple features. PLoS One. 2012;7(12):e50748.
Andresen H, Bier FF. Chapter 8 peptide microarrays for serum antibody diagnostics. In: Bilitewski U, editor. Microchip methods in diagnostics. Humana Press; 2009. p. 123–34.
Pau CP, Luo W, McDougal JS. Chimeric multiple antigenic peptides for simultaneous detection of specific antibodies to HIV-1 groups M, N, O, and HIV-2. J Immunol Methods. 2007;318(1–2):59–64.
Hancock DC, Reilly NJO. Synthetic peptides as antigens for antibody production in methods in molecular biology. 3rd Ed. Burns R, editor. Vol. 295. Humana Press; 2005 .p. 13-25.
Lines JA, Yu Z, Dedkova LM, Chen S. Design and expression of a short peptide as an HIV detection probe. Biochem Biophys Res Commun. 2014;443(1):308–12.
Ponomarenko JV, van Regenmortel MHV. B-Cell epitope prediction BT - Structural bioinformatics. In: Gu J, Bourne PE, editors. Structural bioinformatics second edition. John Wiley and Sons; 2009. p. 849–79.
Potocnakova L, Bhide M, Pulzova LB. An introduction to B-Cell epitope mapping and in silico epitope prediction. J Immunol Res. 2016;2016.
US Food and Drug Administration. Complete list of donor screening assays for infectious agents and HIV diagnostic assays. Infectious Disease Test [Internet]. 2016. Available from: https://www.fda.gov/biologicsbloodvaccines/bloodbloodproducts/approvedproducts/licensedproductsblas/blooddonorscreening/infectiousdisease/ucm080466.htm
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