Effects of Hypoxic Adipose-Derived Mesenchymal Stem Cells and Their Secretome on Type II Collagen Expression in a Rabbit Model of Disc Degeneration
Research
DOI:
https://doi.org/10.55175/cdk.v53i10.2354Kata Kunci:
Ad-MSCs, intervertebral disc degeneration, rabbit model, secretome, type II collagenAbstrak
Introduction: Intervertebral disc (IVD) degeneration is a major cause of low back pain, and is characterized by the loss of extracellular matrix, particularly type II collagen. Regenerative therapies using mesenchymal stem cells (MSCs) and their secretome have shown promise, but their comparative effects on type II collagen expression require further investigation. This study aimed to evaluate and compare the effects of allogeneic hypoxic adipose-derived mesenchymal stem cells (Ad-MSCs) and their secretome on type II collagen expression in a rabbit model of IVD degeneration. Methods: A true experimental study was conducted on 18 New Zealand White rabbits. Degeneration was induced by needle puncture. Subjects were divided into three groups: control (fibrin glue), P1 (hypoxic Ad-MSC), and P2 (secretome). After 8 weeks, type II collagen expression was analyzed using immunohistochemistry. Results: Both P1 and P2 showed significantly higher type II collagen expression than the control (p = 0.001 and p < 0.001, respectively). The secretome group (P2) showed significantly higher expression than the Ad-MSC group (P1) (p = 0.039). These findings suggest that the secretome may provide a greater stimulatory effect on type II collagen expression than direct cell administration. Conclusion: The hypoxic Ad-MSC-derived secretome resulted in higher type II collagen expression than hypoxic Ad-MSCs administration in this rabbit model.
Unduhan
Referensi
Wu A, March L, Zheng X, Huang J, Wang X, Zhao J, et al. Global low back pain prevalence and years lived with disability from 1990 to 2017:estimates from the Global Burden of Disease Study 2017. Ann Transl Med. 2020;8(6):299. doi: 10.21037/atm.2020.02.175.
Oichi T, Taniguchi Y, Oshima Y, Tanaka S, Saito T. Pathomechanism of intervertebral disc degeneration. JOR Spine. 2020;3(1):e1076. doi:10.1002/jsp2.1076.
Waxenbaum JA, Reddy V, Futterman B. Anatomy, back, intervertebral discs [Internet]. Treasure Island (FL): StatPearls Publishing; 2026. Available from: https://www.ncbi.nlm.nih.gov/books/NBK470583/. PMID: 29262063.
Sharabi M, Wade K, Haj-Ali R. The mechanical role of collagen fibers in the intervertebral disc. Biomechanics of the spine: basic concepts, spinal disorders and treatments. Elsevier; 2018. p. 105–23. doi: 10.1016/B978-0-12-812851-0.00007-0.
Wang C, Wang WJ, Yan YG, Xiang YX, Zhang J, Tang ZH, et al. MicroRNAs: new players in intervertebral disc degeneration. Clin Chim Acta. 2015;450:333–41. doi: 10.1016/j.cca.2015.09.011.
Wangler S, Kamali A, Wapp C, Wuertz-Kozak K, Hackel S, Fortes C, et al. Uncovering the secretome of mesenchymal stromal cells exposed to healthy, traumatic, and degenerative intervertebral discs: a proteomic analysis. Stem Cell Res Ther. 2021;12(1):11. doi: 10.1186/s13287-020-02062-2.
Zakrzewski W, Dobrzynski M, Szymonowicz M, Rybak Z. Stem cells: past, present, and future. Stem Cell Res Ther. 2019;10(1):68. doi: 10.1186/s13287-019-1165-5.
Ferreira JR, Teixeira GQ, Santos SG, Barbosa MA, Almeida-Porada G, Gonçalves RM. Mesenchymal stromal cell secretome: influencing therapeutic potential by cellular pre-conditioning. Front Immunol. 2018;9:2837. doi: 10.3389/fimmu.2018.02837.
Vizoso FJ, Eiro N, Cid S, Schneider J, Perez-Fernandez R. Mesenchymal stem cell secretome: toward cell-free therapeutic strategies in regenerative medicine. Int J Mol Sci. 2017;18(9):1852. doi: 10.3390/ijms18091852.
Rumboko RF, Noor Z, Nurikhwan PW, Putera HD, Panghiyangani R. Quality of life of elderly patients with low back pain at Ulin General Hospital Banjarmasin. Cermin Dunia Kedokt. 2025;52(12):789–94. https://doi.org/10.55175/cdk.v52i12.1266.
Istiqomah S, Raharjo W, Fitriangga A. Faktor-faktor yang berhubungan dengan keluhan nyeri punggung bawah supir bus DAMRI. Cermin Dunia Kedokt. 2024;51(3):124–9. https://doi.org/10.55175/cdk.v51i3.1262.
Chiang ER, Ma HL, Wang JP, Chang MC, Liu CL, Chen TH, et al. Use of allogeneic hypoxic mesenchymal stem cells for treating disc degeneration in rabbits. J Orthop Res. 2019;37(6):1440–50. doi: 10.1002/jor.24342.
Gui K, Ren W, Yu Y, Li X, Dong J, Yin W. Inhibitory effects of platelet-rich plasma on intervertebral disc degeneration: a preclinical study in a rabbit model. Med Sci Monit. 2015;21:1368–75. doi: 10.12659/MSM.892510.
Hendrijantini N, Kuntjoro M, Agustono B, Sitalaksmi RM, Ari MDA, Theodora M, et al. Human umbilical cord mesenchymal stem cells induction in peri-implantitis Rattus norvegicus accelerates and enhances osteogenesis activity and implant osseointegration. Saudi Dent J. 2023;35(2):147–53. doi: 10.1016/j.sdentj.2023.01.003.
Lyu FJ, Cui H, Pan H, Cheung KMC, Cao X, Iatridis JC, et al. Painful intervertebral disc degeneration and inflammation: from laboratory evidence to clinical interventions. Bone Res. 2021;9(1):7. doi: 10.1038/s41413-020-00125-x.
Unduhan
Diterbitkan
Cara Mengutip
Terbitan
Bagian
Lisensi
Hak Cipta (c) 2026 Romaniyanto, Dykall Naf’an Dzikri, Daffa Sadewa, Muhammad Setya Fachreza
Artikel ini berlisensi Creative Commons Attribution-NonCommercial 4.0 International License.
